Single Particle Imaging With The Volta Phase Plate

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Nevertheless, the limitations due to reduced signal-to-noise ratio resulting from contrast loss remain. In-focus single particle cryo-EM enabled by the Volta phase plate (VPP) Data acquisition was done using SerialEM software 45 and custom macros for automated single particle data acquisition with Volta phase plate at − 500 nm defocus 14 . SUMMARY Volta phase plate (VPP) is a recently developed trans-mission electron microscope (TEM) apparatus that can significantly enhance the image contrast of biological samples in

Challenges and opportunities in cryo-EM with phase plate

Abstract Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the

Introduction Phase plates are one of the technologies holding promise for future performance improvements in cryo-electron microscopy (cryo-EM). Direct electron detectors already Nevertheless, the limitations due to reduced signal-to-noise ratio resulting from contrast in focus loss remain. In-focus single particle cryo-EM enabled by the Volta phase plate (VPP) holds the We present a method for in-focus data acquisition with a phase plate that enables near-atomic resolution single particle reconstructions. Accurate focusing is the determining

Heating – to ~ 300 oC is essential for maintaining the phase plate in “Volta mode”. It prevents contamination and accelerates the “healing” (~ 1 – 2 days) of the used spots on the PP. Danev, R. & Baumeister, W. Cryo-EM single particle analysis with the Volta phase plate. 142 Elife5(2016). 143 9. Khoshouei, M. et al. Volta phase plate cryo-EM of the small protein

The Volta phase plate enables in-focus single particle analysis at near atomic resolutions, which could expand the capabilities of cryo-EM, especially for small, flexible or The Volta Phase Plate for Single Particle Analysis Initially we were using the VPP in-focus because of the lack of software support for phase plate CTF fitting and correction.

Phase Plates for Transmission Electron Microscopy
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Subtomogram analysis using the Volta phase plate
Max Planck Institute of Biochemistry Martinsried, Germany

Spherical aberration becomes a limiting factor for achieving resolutions below 3 Å with in-focus phase plate images. The phase plate could enable single particle analysis of

ABSTRACT Contrast in electron cryo-microscopy (cryo-EM) is limited by the weak phase and radiation sensitive nature of biologic samples embedded in vitrified ice. We have recently AbstractPreviously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the Previously, we reported an in-focus data acquisition method for cryo-EM single particle analysis with the Volta phase plate (VPP) (Danev and Baumeister, 2016). Here, we extend the

Label-free single-particle imaging approach for ultra-rapid detection ...

In-focus single particle cryo-EM enabled by the Volta phase plate (VPP) holds the promise of yielding up to a twofold boost in signal-to-noise ratio and therefore enhancing our ability to

Abstract Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the SUMMARY Volta phase plate (VPP) is a recently developed trans-mission electron microscope (TEM) apparatus that can significantly enhance the image contrast of biological samples in

Loeffelholz O von, Papai G, Danev R, et al (2018) Volta phase plate data collection facilitates image processing and cryo-EM structure determination. J Struct Biol 202:191–199 Phase plates are a new technique in the field of cryo-electron microscopy. They provide improved contrast and signal-to-noise ratio in images of radiation sensitive specimens. We present a method for in-focus data acquisition with a phase plate that enables near-atomic resolution single particle reconstructions. Accurate focusing is the determining

Abstract 1 Previously, we reported an in-focus data acquisition method for cryo-EM single particle analysis with the 2 Volta phase plate (VPP) (Danev and Baumeister, 2016). Here, we extend Here we demonstrate the benefits of Volta phase plates for single-particle enables near atomic resolution single analysis by time-efficient cryo-EM structure determination of 257 kDa human peroxiredoxin-3 Summary Volta phase plate (VPP) is a recently developed transmission (TEM) apparatus that can significantly enhance the image contrast of biological samples in , and

Challenges and opportunities in cryo-EM with phase plate
Phase Plates for Single Particles
Cryo-EM single particle analysis with the Volta phase plate
Single Particle Imaging with the Volta Phase Plate
Cryo-EM single particle analysis with the Volta phase plate.

The Volta phase plate (VPP) is a remarkably simple device comprising a thin (~10 nm) amorphous carbon film positioned at the back focal plane of the objective lens (Fig. 1) [1]. The film is

Phase plates are a new technique in the field of cryo-electron microscopy. They provide enabled by improved contrast and signal-to-noise ratio in images of radiation sensitive specimens.

Previously, we reported an in-focus data acquisition method for cryo-EM single-particle analysis with the Volta phase plate (Danev and Baumeister, 2016). Here, we extend the technique to Recent developments in new apparatuses, especially the Volta phase plate (VPP), have been increasingly used in cryo-EM. VPP could boost the image contrast significantly and

Building on previous work in cryo-electron microscopy (Entchev et al, 2015), it shown that a combination of the Volta phase plate and a small amount of defocus can simplify

SUMMARY Volta phase plate (VPP) is a recently developed trans-mission electron microscope (TEM) apparatus that can significantly enhance the image contrast of biological samples in

Fan et al. took a unique advantage of the optical system by combining the Volta phase plate and Cs-corrector in a modern TEM to collect high-resolution micrographs of frozen

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Single Particle Imaging With The Volta Phase Plate

Challenges and opportunities in cryo-EM with phase plate